Human T cells loaded with superparamagnetic iron oxide nanoparticles retain antigen-specific TCR functionality

Background Immunotherapy of cancer is an emerging field with the potential to improve long-term survival. Thus far, adoptive transfer tumor-specific T cells represents effective treatment option for tumors hematological system such as lymphoma, leukemia or myeloma. However, in solid tumors, efficacy low owing immunosuppressive microenvironment, on-target/off-tumor toxicity, limited extravasation out blood vessel, ineffective trafficking into tumor region. Superparamagnetic iron oxide nanoparticles (SPIONs) can make magnetically controllable site-specific enrichment. Methods In this study, we investigated influence SPION-loading on primary human targeted cell therapy. For this, analyzed cellular mechanics and response after stimulation via exogenous receptor (TCR) specific melanoma antigen MelanA endogenous TCR cytomegalovirus pp65 compared them that had not received SPIONs. Results showed no mechanics, therefore retaining their ability deform external pressure. Additionally, did impair proliferation, expression activation markers, cytokine secretion, killing antigen-specific mediated by TCR. Conclusion summary, demonstrated affect functionality TCR, which allows future approaches using SPIONs enrichment tumors.